Potensi Aktivitas Antibakteri Fungi Endofit batang parang romang (Boehmeria Virgata (G.Forst.) Guill) Terhadap Bakteri penyebab infeksi mulut dengan metode KLT-AutoBioautografi
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ABSTRACT
ANDI RAFIQAH AULIA. Potential Antibacterial Activity of
Endophytic Fungi of Parang Romang Stem (Boehmeria virgata (G. Forst.)
Guill) Against Bacteria Causing Oral Infection With TLC-Bioautography
Method (Suprvised by Rachmat Kosman and Rusli).
Parang romang plant (Boehmeria virgata (G.Forst.) Guill) is known
to have antibacterial activity. This study aims to determine the antibacterial
activity of endophytic fungal isolates from parang romang stems against
bacteria that cause oral infections using the TLC-Bioautography method.
The research began with the isolation of endophytic fungi and antagonistic
tests, then obtained 2 active isolates against the test bacteria, namely
IFBPR-8 and IFBPR-9. Both isolates were then fermented for 14 days at
room temperature using MYB (Maltose Yeast Broth) medium. The
fermentation results were extracted using ethyl acetate to produce
fermentate extract. The extract obtained was followed by TLC testing using
cloroform:methanol (15:1) eluent. The TLC-Bioautography test of IFBPR-8
isolate obtained Rf values of 0,78 and 0,34, and IFBPR-9 isolate obtained
Rf values of 0,78 and 0,29 which showed antibacterial activity against
bacteria Aggregatibacter actinomycetencomitans, Porphyromonas
gingivalis, Prevotella intermedia, Streptococcus mutas. The identification of
chemical components of endophytic fungal isolates from parang romang
stems is positive for alkaloids and saponins.
Key words: Antibacterial, Boehmeria virgata (G.Forst.) Guill, TLCBioautography
ANDI RAFIQAH AULIA. Potential Antibacterial Activity of
Endophytic Fungi of Parang Romang Stem (Boehmeria virgata (G. Forst.)
Guill) Against Bacteria Causing Oral Infection With TLC-Bioautography
Method (Suprvised by Rachmat Kosman and Rusli).
Parang romang plant (Boehmeria virgata (G.Forst.) Guill) is known
to have antibacterial activity. This study aims to determine the antibacterial
activity of endophytic fungal isolates from parang romang stems against
bacteria that cause oral infections using the TLC-Bioautography method.
The research began with the isolation of endophytic fungi and antagonistic
tests, then obtained 2 active isolates against the test bacteria, namely
IFBPR-8 and IFBPR-9. Both isolates were then fermented for 14 days at
room temperature using MYB (Maltose Yeast Broth) medium. The
fermentation results were extracted using ethyl acetate to produce
fermentate extract. The extract obtained was followed by TLC testing using
cloroform:methanol (15:1) eluent. The TLC-Bioautography test of IFBPR-8
isolate obtained Rf values of 0,78 and 0,34, and IFBPR-9 isolate obtained
Rf values of 0,78 and 0,29 which showed antibacterial activity against
bacteria Aggregatibacter actinomycetencomitans, Porphyromonas
gingivalis, Prevotella intermedia, Streptococcus mutas. The identification of
chemical components of endophytic fungal isolates from parang romang
stems is positive for alkaloids and saponins.
Key words: Antibacterial, Boehmeria virgata (G.Forst.) Guill, TLCBioautography