Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves
Abstrak
Biancaea sappan (BS) is traditionally used to treat anticonvulsants, anti�inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant,
antioxidant, and antimicrobial. Flavonoids are found in Secang; flavonoids
are secondary metabolites that have antioxidant activity. This study aims to
identify the flavonoids using TLC and determination of flavonoids content
in BS leaves. Initially, The methanol extract of BS was obtained by
maceration with ethanol. The qualitative analysis of flavonoid was using
TLC and visualization by sprayed with AlCl3. The determination of total
flavonoid content is conducted based on the AlCl3 method with total
flavonoids expressed in QE (Quercetin equivalent) at the maximum
wavelength of 431 nm. The research results showed that BS leaves contain
flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg
QE/g extract
antioxidant, and antimicrobial. Flavonoids are found in Secang; flavonoids
are secondary metabolites that have antioxidant activity. This study aims to
identify the flavonoids using TLC and determination of flavonoids content
in BS leaves. Initially, The methanol extract of BS was obtained by
maceration with ethanol. The qualitative analysis of flavonoid was using
TLC and visualization by sprayed with AlCl3. The determination of total
flavonoid content is conducted based on the AlCl3 method with total
flavonoids expressed in QE (Quercetin equivalent) at the maximum
wavelength of 431 nm. The research results showed that BS leaves contain
flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg
QE/g extract